بزرگنمایی تصویر

مستر میکس PCR

6،000،000 ﷼1ml

خرید و قیمت مسترمیکس pcr
عملکرد بهینه مسترمیکس پی سی آر امکان تکثیر DNA با غلظت و کیفیت مختلف را فراهم می کند.
مسترمیکس پی سی آر یکتاتجهیز مستر میکس آماده استفاده
بافر بهینه سازی شده و استفاده ازغلظت بافری مناسب جهت افزایش عملکرد واکنش
عدم شکل گیری باندهای غیراختصاصی در واکنش PCR
بازدهی بالای واکنش پی سی آر محصولات واکنش قابل
استفاده در سایر کاربردهای مولکولی

شناسه محصول: YT1551 دسته ها: PCR, آنزیم ها و مستر میکس, محصولات

توضیحات

مستر میکس PCR master mix | PCR

مستر میکس چیست؟ مستر میکس PCR شرکت یکتا تجهیز آزما (YTA PCR mastermix) یک محلول آماده استفاده حاوی Taq DNA پلیمراز، dNTPs ، MgCl2 و بافر با غلظت مناسب برای واکنش PCR می باشد.

برای انجام واکنش با استفاده از مسترمیکس pcr شما فقط باید پرایمرها و نمونه را به مخلوط واکنش اضافه کنید.

مسترمیکس پی‌سی‌آر شرکت یکتا تجهیز آزما با غلظت 2X عرضه می‌شود و کاربران باید آن را به غلظت نهایی 1X رقیق کنند. این مستر میکس با کاهش خطای انسانی، کاهش زمان آماده‌سازی واکنش و احتمال آلودگی، به طور قابل توجهی عملکرد آزمایش را بهبود می‌بخشد.علاوه بر این حاوی یک رنگ آبی برای انجام الکتروفورز پس از PCR است ، بنابراین نیازی به خرید و استفاده از لودینگ بافر جداگانه برای انجام الکتروفورز نمی باشد و می توانید به سادگی بخشی از محصول واکنش را روی ژل آگارز قرار دهید.

«این مسترمیکس انتخابی عالی برای انجام واکنش PCR با دقت بالا است.»

برای اطلاع از مزایای مسترمیکس پی سی آر ( PCR mastermix ) کلیک کنید

کاربرد PCR mastermix :

انجام واکنش زنجیره ای پلیمراز بر روی نمونه های DNA
تکثیر نمونه های DNA برای سایر کاربردهای مولکولی

ویژگی های مستر میکس PCR :

خرید و قیمت مستر میکس pcr
عملکرد بهینه مسترمیکس پی سی آر امکان تکثیر DNA با غلظت و کیفیت مختلف را فراهم می کند.
مستر میکس PCR شرکت یکتا تجهیز آزما آماده مصرف می باشد
این محصول بافر بهینه‌سازی‌شده‌ای دارد و از غلظت بافری مناسبی برای افزایش عملکرد واکنش استفاده می‌کند.
عدم شکل گیری باندهای غیراختصاصی در واکنش PCR
بازدهی بالای واکنش پی سی آر محصولات قابل واکنش
استفاده در سایر کاربردهای مولکولی

مسترمیکس PCR یا مسترمیکس سوپر PCR یا مسترمیکس Pfu ؟ کدام انتخاب مناسب تر است ؟

انتخاب نوع آنزیم پلیمراز برای واکنش PCR باید بر اساس هدف نهایی آزمایش تعیین شود. در صورتی‌ که هدف از PCR صرفاً تأیید حضور یک توالی خاص یا انجام آزمایش‌های تشخیصی باشد، استفاده از مستر میکس‌های معمولی حاوی Taq DNA polymerase به دلیل سرعت بالا، هزینه‌ کمتر و راندمان مناسب توصیه می‌شود. با این حال، برای کاربردهایی که در آن‌ها دقت توالی اهمیت حیاتی دارد ـ از جمله کلونینگ دقیق، طراحی سازه‌های نوترکیب، یا آماده‌سازی نمونه برای توالی‌یابی ـ استفاده از آنزیم‌های با fidelity بالا مانند Pfu DNA polymerase به دلیل دارا بودن فعالیت proofreading و نرخ خطای پایین‌تر توصیه می‌شود. در مجموع، تطابق نوع آنزیم با هدف آزمایش، نقش کلیدی در کیفیت و صحت نتایج PCR خواهد داشت.
مسترمیکس PCR یکتاتجهیز آزما حاوی آنزیم Taq DNA polymerase میباشد که برای استفاده کاربر مقرون بصرفه بوده ، در مقابل مسترمیکس سوپر PCR حاوی دو آنزیم Taq DNA polymerase و Pfu و همچنین مسترمیکس Pfu شامل فقط آنزیم Pfu میباشد که کاربر بر اساس هدف آزمایش میتواند یکی از این سه نوع مسترمیکس را انتخاب کند.

دستورالعمل ها

دانلود دستورالعمل مستر میکس PCR master mix | PCR

سوالات متداول

ژورنالهای علمی و پژوهشی

Published online 2019 Sep| Turk J Gastroenterol | Read article

Anti-colon cancer activity of Bifidobacterium metabolites on colon cancer cell line SW742

DNA of cell line extraction was performed by using a high pure DNA isolation kit (Yekta Tajhiz Azma, Tehran, Iran) according to the manufacturer’s instructions. After centrifugation, the cell genome pellet was visible at the ... READ MORE

Published online 2023 Mar 7| Cell J| Read article

Investigating The Correction of IVS II-1 (G> A) Mutation in HBB Gene in TLS-12 Cell Line Using CRISPR/Cas9 System

DNA was extracted from homogeneous cells with a Yekta Tajhiz DNA extraction kit (FABGK001, Iran). Then, the HBB gene region of each of the TLS-12 single clones was amplified with PCR reaction. ... READ MORE

Published online 2019 Aug 12| Heliyon| Read article

Identification of novel Glutathione S-Transferases epsilon 2 mutation in Anopheles maculipennis s.s. (Diptera: Culicidae)

The PCR reactions were performed in a final volume of 25μl, containing a 12.5μl master mix (Yekta Tajhiz Azma, Tehran, Iran), 1μl of each primer, 8.5μl ddH₂O and 2μl of genomic DNA... READ MORE

Published online 2019 Aug 12| BMC Res Notes| Read article

Ribosomal DNA internal transcribed spacer 2 sequence analysis and phylogenetic comparison of seven cockroach species in northwestern Iran

PCR reactions of ITS2 fragment were performed in a total volume of 25 µL master mix. Each reaction contained 2 µL genomic DNA, 12.5 μL PCR Master Mix (Yekta Tajhiz Azma, Tehran, Iran), 0.2 μL Taq polymerase, 1 μL each primer ... READ MORE

Published online2023| | Read article

Genetic predisposition to nonalcoholic fatty liver disease: insights from ANGPTL8 gene variants in Iranian adults

The ARMS-PCR technique was employed to separate alleles at rs892066 (C/G). ARMS-PCR experiments were carried out in two tubes using a PCR kit (Yekta Tajhiz Azma, Iran) and the following primersr ... READ MORE

|Published online 2023 Apr 6 | IJID Reg| Read article

Evaluation of the prevalence of bovine leukemia virus DNA in peripheral blood mononuclear cells of multiple sclerosis patients

The mixture for β-actin, BLV Gag, and BLV Tax included 12 µL of the commercially available 2X Super MasterMix (Yekta Tajhiz Azma, Iran), 1 µL of each gene-specific forward ... READ MORE

Published online 2022 Dec 31 | Arch Razi Inst | Read article

Detection of Hepatocystis sp. Infection in Vervet Monkeys (Chlorocebus Pygrythrus) Imported from Tanzania Using Molecular and Microscopic Methods

Each PCR contained DNA, 10 pmole forward and reverse primers, as well as2X PCR Super Master Mix (Yekta Tajhiz Azma, Iran). Amplification used cycles of 95°C for ... READ MORE

Published online 2023 Sep 7| Lipids Health Dis | Read article

Genetic predisposition to nonalcoholic fatty liver disease: insights from ANGPTL8 gene variants in Iranian adults

ARMS-PCR experiments were carried out in two tubes using a PCR kit (Yekta Tajhiz Azma, Iran) and the following primers ... READ MORE

Published online 2023 Oct 21 | Vet Med Sci | Read article

Effects of wheat bran replacement with pomegranate seed pulp on rumen fermentation, gas production, methanogen and protozoa populations of camel and goat rumen using competitive PCR technique: An in vitro study

Master Mix 2x (Yekta Tajhiz Azma), 10 pM as a forward primer, 10 pM as a reverse primer and 8.5 μL distilled water. In order to distinguish suitable dilutions for the counting population, the PCR products were analysed by running on ... READ MORE

Published online 2022 Dec 20 | Springer Nature | Read article

Targeted integration in CHO cells using CRIS-PITCh/Bxb1 recombinase–mediated cassette exchange hybrid system

for 10 min. Out-out PCR was carried out using Super PCR Master Mix 2x (Yekta Tajhiz Azma Co., Iran) according to the following PCR program: 95 °C for 5 min; 30 × : 95 °C for 30 s, 59 °C for 30 s, 72 °C for 3 min and 30 ... READ MORE

Published online 2023 Nov 6 |BMC Infect Dis | Read article

Molecular diagnosis, phylogenetic analysis, and antifungal susceptibility profiles of Candida species isolated from neutropenic oncological patients

The PCR amplification was done in 25 µl reaction volumes, including 1 µl of each forward and reverse primer, 12.5 µl of super PCR master mix (Yekta Tajhiz Azma, Iran), 3 µl of DNA template, and 7.5 µl of deionized distilled ... READ MORE

Published online 2023 Jun 5| Vet Med Sci | Read article

Brucella abortus antigen omp25 vaccines: Development and targeting based on Lactococcus lactis

The PCR reaction was conducted to monitor omp25 using specific primers (Table 1). A 20‐mL PCR reaction contains 2 mL of ×10 PCR buffer (Yekta Tajhiz Azma, Iran), 2 mM MgCl₂, 200 μM dNTPs (Yekta Tajhiz Azma, Iran), 10 pmol of each primer ... READ MORE

Published online 2023 Jul 22| J Insect Sci | Read article

Molecular analysis of acetylcholinesterase gene in field-collected populations of Musca domestica (Diptera: Muscidae) in Northwestern Iran

PCR reactions contained 1 μl of genomic DNA, 1 μl of each primer, 9.5 μl of ddH₂O, and 12.5 μl of Master Mix (Yekta Tajhiz Azma). PCR amplifications were set up in a hot start at 94 °C for 3 min, followed by 40 cycles of denaturation at 95 °C for 30 s, annealing a ... READ MORE

Published online 2021 Feb 6| Mol Genet Genomic Med | Read article

The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran

The microtube's content was as follows: 12.5 μl of Commercial 2X Master Mix (Yekta Tajhiz Azma, Iran), at final concentration of 500 nanomolar for each of the Forward primer ... READ MORE

مستر میکس PCR

مستر میکس PCR master mix | PCR

کاربرد PCR mastermix :

ویژگی های مستر میکس PCR :

مسترمیکس PCR یا مسترمیکس سوپر PCR یا مسترمیکس Pfu ؟ کدام انتخاب مناسب تر است ؟

دستورالعمل ها

سوالات متداول

ژورنالهای علمی و پژوهشی

Published online 2019 Sep| Turk J Gastroenterol | Read article

Anti-colon cancer activity of Bifidobacterium metabolites on colon cancer cell line SW742

DNA of cell line extraction was performed by using a high pure DNA isolation kit (Yekta Tajhiz Azma, Tehran, Iran) according to the manufacturer’s instructions. After centrifugation, the cell genome pellet was visible at the ... READ MORE

Published online 2023 Mar 7| Cell J| Read article

Investigating The Correction of IVS II-1 (G> A) Mutation in HBB Gene in TLS-12 Cell Line Using CRISPR/Cas9 System

DNA was extracted from homogeneous cells with a Yekta Tajhiz DNA extraction kit (FABGK001, Iran). Then, the HBB gene region of each of the TLS-12 single clones was amplified with PCR reaction. ... READ MORE

Published online 2019 Aug 12| Heliyon| Read article

The PCR reactions were performed in a final volume of 25μl, containing a 12.5μl master mix (Yekta Tajhiz Azma, Tehran, Iran), 1μl of each primer, 8.5μl ddH2O and 2μl of genomic DNA... READ MORE

Published online 2019 Aug 12| BMC Res Notes| Read article

Ribosomal DNA internal transcribed spacer 2 sequence analysis and phylogenetic comparison of seven cockroach species in northwestern Iran

PCR reactions of ITS2 fragment were performed in a total volume of 25 µL master mix. Each reaction contained 2 µL genomic DNA, 12.5 μL PCR Master Mix (Yekta Tajhiz Azma, Tehran, Iran), 0.2 μL Taq polymerase, 1 μL each primer ... READ MORE

Published online2023| | Read article

Genetic predisposition to nonalcoholic fatty liver disease: insights from ANGPTL8 gene variants in Iranian adults

The ARMS-PCR technique was employed to separate alleles at rs892066 (C/G). ARMS-PCR experiments were carried out in two tubes using a PCR kit (Yekta Tajhiz Azma, Iran) and the following primersr ... READ MORE

|Published online 2023 Apr 6 | IJID Reg| Read article

Evaluation of the prevalence of bovine leukemia virus DNA in peripheral blood mononuclear cells of multiple sclerosis patients

The mixture for β-actin, BLV Gag, and BLV Tax included 12 µL of the commercially available 2X Super MasterMix (Yekta Tajhiz Azma, Iran), 1 µL of each gene-specific forward ... READ MORE

Published online 2022 Dec 31 | Arch Razi Inst | Read article

Detection of Hepatocystis sp. Infection in Vervet Monkeys (Chlorocebus Pygrythrus) Imported from Tanzania Using Molecular and Microscopic Methods

Each PCR contained DNA, 10 pmole forward and reverse primers, as well as2X PCR Super Master Mix (Yekta Tajhiz Azma, Iran). Amplification used cycles of 95°C for ... READ MORE

Published online 2023 Sep 7| Lipids Health Dis | Read article

Genetic predisposition to nonalcoholic fatty liver disease: insights from ANGPTL8 gene variants in Iranian adults

ARMS-PCR experiments were carried out in two tubes using a PCR kit (Yekta Tajhiz Azma, Iran) and the following primers ... READ MORE

Published online 2023 Oct 21 | Vet Med Sci | Read article

Effects of wheat bran replacement with pomegranate seed pulp on rumen fermentation, gas production, methanogen and protozoa populations of camel and goat rumen using competitive PCR technique: An in vitro study

Master Mix 2x (Yekta Tajhiz Azma), 10 pM as a forward primer, 10 pM as a reverse primer and 8.5 μL distilled water. In order to distinguish suitable dilutions for the counting population, the PCR products were analysed by running on ... READ MORE

Published online 2022 Dec 20 | Springer Nature | Read article

Targeted integration in CHO cells using CRIS-PITCh/Bxb1 recombinase–mediated cassette exchange hybrid system

for 10 min. Out-out PCR was carried out using Super PCR Master Mix 2x (Yekta Tajhiz Azma Co., Iran) according to the following PCR program: 95 °C for 5 min; 30 × : 95 °C for 30 s, 59 °C for 30 s, 72 °C for 3 min and 30 ... READ MORE

Published online 2023 Nov 6 |BMC Infect Dis | Read article

Molecular diagnosis, phylogenetic analysis, and antifungal susceptibility profiles of Candida species isolated from neutropenic oncological patients

The PCR amplification was done in 25 µl reaction volumes, including 1 µl of each forward and reverse primer, 12.5 µl of super PCR master mix (Yekta Tajhiz Azma, Iran), 3 µl of DNA template, and 7.5 µl of deionized distilled ... READ MORE

Published online 2023 Jun 5| Vet Med Sci | Read article

Brucella abortus antigen omp25 vaccines: Development and targeting based on Lactococcus lactis

The PCR reaction was conducted to monitor omp25 using specific primers (Table 1). A 20‐mL PCR reaction contains 2 mL of ×10 PCR buffer (Yekta Tajhiz Azma, Iran), 2 mM MgCl2, 200 μM dNTPs (Yekta Tajhiz Azma, Iran), 10 pmol of each primer ... READ MORE

Published online 2023 Jul 22| J Insect Sci | Read article

Molecular analysis of acetylcholinesterase gene in field-collected populations of Musca domestica (Diptera: Muscidae) in Northwestern Iran

PCR reactions contained 1 μl of genomic DNA, 1 μl of each primer, 9.5 μl of ddH2O, and 12.5 μl of Master Mix (Yekta Tajhiz Azma). PCR amplifications were set up in a hot start at 94 °C for 3 min, followed by 40 cycles of denaturation at 95 °C for 30 s, annealing a ... READ MORE

Published online 2021 Feb 6| Mol Genet Genomic Med | Read article

The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran

The microtube's content was as follows: 12.5 μl of Commercial 2X Master Mix (Yekta Tajhiz Azma, Iran), at final concentration of 500 nanomolar for each of the Forward primer ... READ MORE

محصولات مرتبط

Yekta Tajhiz Azma

The PCR reactions were performed in a final volume of 25μl, containing a 12.5μl master mix (Yekta Tajhiz Azma, Tehran, Iran), 1μl of each primer, 8.5μl ddH₂O and 2μl of genomic DNA... READ MORE

The PCR reaction was conducted to monitor omp25 using specific primers (Table 1). A 20‐mL PCR reaction contains 2 mL of ×10 PCR buffer (Yekta Tajhiz Azma, Iran), 2 mM MgCl₂, 200 μM dNTPs (Yekta Tajhiz Azma, Iran), 10 pmol of each primer ... READ MORE

PCR reactions contained 1 μl of genomic DNA, 1 μl of each primer, 9.5 μl of ddH₂O, and 12.5 μl of Master Mix (Yekta Tajhiz Azma). PCR amplifications were set up in a hot start at 94 °C for 3 min, followed by 40 cycles of denaturation at 95 °C for 30 s, annealing a ... READ MORE