محلول ترایزول | وایتیزول | YTzol Pure RNA
6،000،000 ﷼ – 22،000،000 ﷼
- YTzol pure RNA reagent
- قیمت ترایزول (وایتیزول ) نسبت به کیت های استخراج RNA که تجاری شده اند بسیار مناسب تر است.
- محلول وایتیزول YTzol مشابه همان ترایزول TRIzol است که با نام تجاری شرکت یکتا تجهیز آزما عرضه می گردد.
- وایتیزول (ترایزول ) قابل استفاده جهت استخراج RNA از طیف وسیعی از نمونه ها می باشد.
- قابلیت استخراج RNA ، DNA یا پروتئین از یک نمونه واحد
- امکان استخراج RNA از نمونه های بافی کوت با استفاده از دستورالعمل مجزا
- دارای دستورالعمل اختصاصی و مجزا جهت استخراج miRNA و RNA از نمونه های خون کامل
- جهت دانلود پروتکل وایتیزول | ترایزول از تب دستورالعمل ها در زیر همین محصول اقدام کنید و جهت خرید وایتیزول|خرید ترایزول از طریق همین صفحه سفارش خود را ثبت نمایید.
| برند |
یکتا تجهیز آزما YTA |
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محلول ترایزول (وایتیزول) YTzol Pure RNA
محلول ترایزول (با برند تجاری YTzol pure RNA شرکت یکتا تجهیز آزما ) یک معرف آماده استفاده بر پایه فنول-گوانیدین است که به منظور جداسازی RNA با کیفیت بالا از انواع نمونه های بیولوژیکی طراحی شده است.
این معرف، یک محلول تک فازی از فنول، گوانیدین ایزوتیوسیانات و سایر اجزای اختصاصی است که جداسازی انواع RNA با اندازه های مولکولی بزرگ یا کوچک را تسهیل می کند.
محلول YTzol برای جداسازی چندین هدف مولکولی ساخته شده بنابراین این امکان را به شما می دهد تا علاوه برRNA ، DNA و پروتئین ها را نیز از یک نمونه واحد استخراج کنید.
این معرف به دلیل مهار بسیار مؤثر فعالیت RNase ، می تواند یکپارچگی RNA را در طول همگن سازی یا لیز نمونه حفظ کند، در حالی که سلول ها کاملا لیز می شوند .با استفاده از معرف YTzol می توانید از مقدار کمی بافت (50-100 میلی گرم) و سلول ، ریبونوکلئیک اسید (RNA) با غلظت بالا استخراج کنید. همچنین سادگی روش این معرف، امکان پردازش همزمان تعداد زیادی از نمونه ها را فراهم می کند.
برای دانلود پروتکل ترایزول از tab دستورالعمل ها می توانید آن را دانلود کنید.
کاربرد:
- ترایزول یا همان وایتیزول با برند یکتاتجهیزآزما قابل استفاده برای استخراج از انواع نمونه های گیاهی،جانوری ، انسانی، مخمر ، باکتری و کشت سلول
- قابل استفاده برای استخراج miRNA از نمونه های خون کامل
- قابل استفاده جهت استخراج RNA از بافی کوت
- امکان استفاده از RNA استخراج شده در واکنش های پایین دستی نظیر PCR
ویژگی ها :
- قیمت ترایزول (وایتیزول ) نسبت به کیت های استخراج RNA که تجاری شده اند بسیار مناسب تر است.
- محلول وایتیزول YTzol مشابه همان ترایزول TRIzol است که با نام تجاری شرکت یکتا تجهیز آزما عرضه می گردد.
- وایتیزول (ترایزول ) قابل استفاده جهت استخراج RNA از طیف وسیعی از نمونه ها می باشد.
- قابلیت استخراج RNA ، DNA یا پروتئین از یک نمونه واحد
- امکان استخراج RNA از نمونه های بافی کوت با استفاده از دستورالعمل مجزا
- دارای دستورالعمل اختصاصی و مجزا جهت استخراج miRNA و RNA از نمونه های خون کامل
- جهت دانلود پروتکل وایتیزول | ترایزول از تب دستورالعمل ها در زیر همین محصول اقدام کنید و جهت خرید وایتیزول|خرید ترایزول از طریق همین صفحه سفارش خود را ثبت نمایید.
دستورالعمل ها
دانلود دستورالعمل محلول YTzol Pure RNA
سوالات متداول
در استخراج توسط YTzol تناسب بین اندازه نمونه و مقدار YTzol بسیار حائز اهمیت است.توصیه می شود در این موارد به مقدار مناسب از معرف YTzol برای نمونه خود دست یابید . به طور عمومی مقدار YTzol سه برابر مقدار نمونه اولیه است اما این مقدار بر اساس نوع نمونه قابل تغییر است. بنابراین سعی کنید تا به مقدار متناسب با نمونه خود دست یابید. از آنجایی که در روش استخراج با YTzol مقداری RNA از دست می رود پیشنهاد می شود به دلیل کم بودن مقدار ماده ژنتیکی نمونه و نیز امکان از دست رفتن همان مقدار اندک طی مراحل شستشو ،جهت انجام چنین استخراجی از ترکیب YTzol با کیت ستونی شرکت FAVORGEN استفاده کنید. برخی از نمونه ها به دلیل ماهیت خود دارای فنول می باشند که استخراج با YTzol کار را سخت میکند. توصیه می شود در مشاهده آلودگی فنول تعداد دفعات شستشو با الکل 70 درصد را افزایش دهید. دقت شود ممکن است مقداری از غلظت RNA با افزایش دفعات شستشو کاهش یابد. از طرفی به میزان YTzol اضافه شده و نیز به مدت زمان انکوباسیون نمونه با YTzol نیز توجه شود . در استخراج با روش ستونی به دلیل استفاده از ستون های با کیفیت سیلیکایی و نیز جایگزین شدن بافرهایی با پایه نمک به جهت لیز نمونه با معرف YTzol که مبتنی بر فنول-گوانیدین می باشد، RNA با کیفیت بالاتری حاصل می شود. در واقع چون معرف YTzol pure RNA یک ماده لیزکننده می باشد ؛ بنابراین برای تمامی نمونه ها بکار برده می شود.
ژورنالهای علمی و پژوهشی
Total RNA was isolated from whole blood samples of BC patients and healthy controls according to the manufacturer instructions, using Trizol (Cat YT9066-YT9065-YT9064, Yekta Tajhiz Azma, Iran). The xylene-ethanol technique was used...READ MORE Total RNA was isolated from whole blood samples of BC patients and healthy controls according to the manufacturer instructions, using Trizol (Cat YT9066-YT9065-YT9064, Yekta Tajhiz Azma, Iran). The xylene-ethanol technique was used...READ MORE Total RNA was extracted from wild-type and knockout cells using the Y-Tizol RNA extraction Kit (Yekta-Tajhiz, Iran). The quality and variety of the collected RNA were assessed using a 2000 Nanodrop spectrometer (Thermo Scientific, USA). 1 µg of total RNA...READ MORE The prepared tissue was stored at − 80 °C. Total RNA extraction was then performed using the TRIzol reagent kit (YeKta Tajhiz Azma kit, Cat. No. YT9065, Tehran, Iran) according to the manufacturer's instructions...READ MORE The total RNA of frozen tissue samples was isolated using the Trizole (Yekta Tajhiz, Iran) according to the manufacturer’s instructions. The quantity and purity of the RNA samples...READ MORE Fifty mg of testicular tissue was homogenized with 1 ml of TRIzol reagent (Yekta Tajhiz Azma, Iran). The RNA concentration and purity were evaluated by reading the absorbance at 230 nm, 260 nm, and 280 nm with a NanoDrop1000 spectrophotometer. The cDNA ...READ MORE Gene expression analysis was performed on CAFs and PBMCs before and in treatment with Curcumin. Total cellular RNA was extracted using the Trizol technique (Yekta Tajhiz Azma, Tehran, Iran). Random hexamer-primed reverse transcription (Yekta Tajhiz Azma Tehran, Iran) was performed on...READ MORE The total RNA from each sample was extracted using Trizol Reagent (YTzole, Yekta Tajhiz Co. Tehran, Iran) according to the manufacturer’s protocol. The NanoDrop™ 2000/2000c Spectrophotometers (Termo Fisher Scientific, USA) were ...Read More Total RNA was extracted from GCs derived from PCOS women and control group using TRizol reagents (Yekta Tajhiz Azma, Iran) as per the manufacturer's recommendations. The quality of the RNA ...Read More Total RNA extraction was then performed using the TRIzol reagent kit (YeKta Tajhiz Azma kit, Cat. No. YT9065, Tehran, Iran) according to the manufacturer's instructions...Read More YTzol Pure RNA (Yekta Tajhiz Azma, YT9066) was used to isolate total RNA from untreated (control) and treated cells with 100 µg/mL ZME, 10 nM Dox and ZME/ Dox combination (100 µg/mL ZME and 10 nM Dox). Quantity of RNA..Read More YTzol Pure RNA (Yekta Tajhiz Azma, YT9066) was used to isolate total RNA from untreated (control) and treated cells with 100 µg/mL ZME, 10 nM Dox and ZME/ Dox combination (100 µg/mL ZME and 10 nM Dox). Quantity of RNA..Read More The prepared tissue was stored at − 80 °C. Total RNA extraction was then performed using the TRIzol reagent kit (YeKta Tajhiz Azma kit, Cat. No. YT9065, Tehran, Iran) according to the manufacturer's instructions...Read More To validate several DEp53TGs involved in apoptosis, qRT-PCR was performed. Total RNA was extracted from GCs derived from PCOS women and control group using TRizol reagents (Yekta Tajhiz Azma, Iran) as per the manufacturer's recommendations...Read More Published on 30 jaunary 2024 |scientifice reports | Read article
Comparison of cytotoxicity of Miltefosine and its niosomal form on chick embryo model
At first, the total RNA of YSM vasculature was extracted by triazole (YTzol Pure RNA, Iran), and cDNA was synthesized by using the (YTzol pure RNA, Yekta Tajhiz, Iran)......READ MORE
Published on 20 November 2024 |scientifice reports | Read article
Comprehensive analysis identifies endocrine fibroblast growth factors as promising prognostic markers for colorectal carcinoma
We used TRIzol reagent to extract total RNA from 116 tissue samples (56 polyps, 30 CRC, and 30 normal colon mucosa) (YTzol pure RNA, Yekta Tajhiz, Iran)......READ MORE
Published on 24 August 2024 |scientifice reports | Read article
Chemical composition of essential and fixed oils of Tagetes erecta fruits (Iran) and their implications in inhibition of cancer signaling
Total RNA was extracted from U87 MG cell lines using the YTzol Reagent (Yekta Tajhiz Azma, Iran) according to the manufacturer’s protocol.....READ MORE
Published on |scientifice reports | Read article
Bilirubin, a hepatoprotective agent that activates SIRT1, PGC-1α, and PPAR-α, while inhibiting NF-κB in rats with metabolic-associated fatty liver disease
Initially, the frozen liver tissues (50–100 mg) were homogenized using a Potter–Elvehjem homogenizer (Bodine Electric, Chicago, IL, USA) and total RNA was extracted using the YTzol extraction solution (Yekta Tajhiz Azma, Iran; Cat. No. YT9065)....READ MORE
Published online 2022 Apr 27 | Cell J | Read article
Down-Regulated Expression of Cystathionine β-Synthase and Cystathionine γ-Lyase in Varicocele, and Infertile Men: A Case-Control Study
The remaining semen samples were washed twice with phosphate-buffered salin (PBS, Sama Tashkhis, Iran) and used for total RNA extraction using YTzol pure RNA (Yekta Tajhiz Azama, Iran) according to the manufacturer’s protocol. cDNA was synthesized from...READ MORE
Published online 2023 Mar | Iran J Basic Med Sci | Read article
Cannabidiol interacts with the FXR/Nrf2 pathway and changes the CB1/CB2 receptors ratio in gentamicin-induced kidney injury in rats
The RNA then was extracted using Trizol (Yekta Tajhiz Azma Co Iran) based on the manufacturer’s instructions. Then the cDNA synthesis was performed using a Reverse Transcriptase cDNA synthesis kit (Yekta Tajhiz Azma Co Iran), based on the protocol. Expression of ...READ MORE
Published online 2022 May-Jun | Avicenna J Phytomed | Read article
Effect of turmeric extract on bone healing in an experimental model of femoral bone fracture
The RNA then was extracted using Trizol (Yekta Tajhiz Azma Co Iran) based on the manufacturer’s instructions. Then the cDNA synthesis was performed using a Reverse Transcriptase cDNA synthesis kit (Yekta Tajhiz Azma Co Iran), based on the protocol. Expression of ...READ MORE
Published online 2023 Mar 23 | Microorganisms | Read article
Candida spp. DNA Extraction in the Age of Molecular Diagnosis
The steel-bullets were prewashed with a lysis buffer and added together with the samples in a cylinder to perform the DNA extraction. After this step, each series of samples was purified according to the described protocol with either phenol-chloroform [66] or a commercial kit (Yekta Tajhiz Azma, Iran).The ....READ MORE
Published online 2023 Nov 6. | Scientific Reports | Read article
The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice
Fifty mg of testicular tissue was homogenized with 1 ml of TRIzol reagent (Yekta Tajhiz Azma, Iran). The RNA concentration and purity were evaluated by reading the absorbance at 230 nm, 260 nm, and 280 nm with a NanoDrop1000 spectrophotometer...READ MORE
Published online 2023 Oct 9 | Cell J | Read article
A Pleurocidin-Like Peptide from Poecilia Mexicana Fish Induces Selective Cytotoxicity in Leukemia Jurkat Cells Through The Apoptosis Pathway
The TRIzol reagent was used to extract total RNA from cultivated cells in each group (Yekta Tajhiz Azma, Iran). Gel electrophoresis and the Nanodrop spectrometer were used to assess the RNA quality and quantity. Complementary DNA was produced according to the manufacturer’s instructions using a random hexamer primer and the reverse transcriptase....READ MORE
Published online 2023 Feb 14 | Cell J | Read article
Aberrant Expression of TET2 Accounts for DNA Hypomethylation in Varicocele
To isolate total RNA, 50 mg of rat testicular tissue, stored at -80°C, was homogenized with 1 ml of TRIzol reagent (Yekta Tajhiz Azma, Iran). The RNA quality and concentration...READ MORE
Published online 2023 Oct 9 | Cell J | Read article
Neutralizing tumor-related inflammation and reprogramming of cancer-associated fibroblasts by Curcumin in breast cancer therapy
Gene expression analysis was performed on CAFs and PBMCs before and in treatment with Curcumin. Total cellular RNA was extracted using the Trizol technique (Yekta Tajhiz Azma, Tehran, Iran). Random hexamer-primed reverse transcription (Yekta Tajhiz Azma Tehran, Iran) was performed... READ MORE
Published online 2023 Jan 11 | Cell Prolif| Read article
The beneficial effects of chick embryo extract preconditioning on hair follicle stem cells: A promising strategy to generate Schwann cells
Total RNA was extracted from cells grown in 6‐well plates at ~90% confluency, using YTzol (Cat. No: YT9063; Yekta Tajhiz Azma, Iran) reagent by chloroform extraction and... READ MORE
Published online2023 | Iran J Basic Med Sci | Read article
Role of cineole in alleviation of acute kidney injury and renal function recovery following gentamicin administration in rats
Total RNA was extracted from cells grown in 6‐well plates at ~90% confluency, using YTzol (Cat. No: YT9063; Yekta Tajhiz Azma, Iran) reagent by chloroform extraction and ethanol precipitation, according to the manufacturer's instructions...READ MORE
Published online 2023 Mar 15 | BMC Genomics | Read article
MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces
Total RNA was extracted from in vivo and each replicate of in vitro samples at different time points using YTzol Pure RNA (Yekta Tajhiz Azma, Iran), according to... READ MORE
Published online 2023 Oct | Scientific Reports | Read article
Identification of GUCA2A and COL3A1 as prognostic biomarkers in colorectal cancer by integrating analysis of RNA-Seq data and qRT-PCR validation
The total RNA from each sample was extracted using Trizol Reagent (YTzole, Yekta Tajhiz Co. Tehran, Iran) according to the manufacturer’s protocol. The NanoDrop™ 2000/2000c Spectrophotometers (Termo Fisher Scientific, USA) were used for...READ MORE
Published online 2023 Jul 5 | Breast Cancer (Auckl) | Read article
Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast Cancer
Published online 2023 Jul 5 | Breast Cancer (Auckl) | Read article
Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast Cancer
Published online 2023 Nov 27 | BMC Cancer | Read article
Genome editing approaches with CRISPR/Cas9: the association of NOX4 expression in breast cancer patients and effectiveness evaluation of different strategies of CRISPR/Cas9 to knockout Nox4 in cancer cells
Published online 2023 Aug 12 | Scientific Reports| Read article
Identification of LncPVT1 and CircPVT1 as prognostic biomarkers in human colorectal polyps
Published online 2023 Apr | Iran J Basic Med Sci| Read article
Chlorogenic acid attenuates liver apoptosis and inflammation in endoplasmic reticulum stress-induced mice
Published online 15 January 2024 | sciencedirect| Read article
The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice
Published online 26 November 2023 | scientific reports| Read article
Neutralizing tumor-related inflammation and reprogramming of cancer-associated fibroblasts by Curcumin in breast cancer therapy
Published online 10 October 2023 | scientific reports| Read article
Identification of GUCA2A and COL3A1 as prognostic biomarkers in colorectal cancaer by integrating analysis of RNA-Seq data and qRT-PCR validation
Published 19 July 2023 | scientific reports| Read article
Evaluation of the p53 pathway in polycystic ovarian syndrome pathogenesis and apoptosis enhancement in human granulosa cells through transcriptome data analysis
Published 12 August 2023 | scientific reports| Read article
Identification of LncPVT1 and CircPVT1 as prognostic biomarkers in human colorectal polyps
Published 25 July 2022 | scientific reports| Read article
Promising anti-leukemic effect of Zataria multiflora extract in combination with doxorubicin to combat acute lymphoblastic leukemia cells (Nalm-6) (in vitro and in silico)
Published | scientific reports| Read article
The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice
Published 12 August 2023| scientific reports| Read article
Identification of LncPVT1 and CircPVT1 as prognostic biomarkers in human colorectal polyps
Published 12 August 2023| scientific reports| Read article
Evaluation of the p53 pathway in polycystic ovarian syndrome pathogenesis and apoptosis enhancement in human granulosa cells through transcriptome data analysis
محصولات مرتبط
راه های ارتباطی
تهران- بزرگراه رسالت – بین بزرگراه باقری و خیابان رشید پلاک 218- ساختمان روتانو- طبقه 9 – واحد 21
نماد اعتماد
یکتا تجهیز آزما یک شرکت تولیدی تحقیقاتی و بازرگانی است که فعالیت خود را از سال 1391 در حیطه ی بیولوژی مولکولی با بهره گیری از متخصصان و اساتید این حوزه آغاز کرد.هدف ما ارائه محصولاتی با کیفیت رقابتی و با استانداردهای جهانی است.تمامی حقوق برای یکتا تجهیزآزما محفوظ می باشد.
