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مسترمیکس ریل تایم سایبرگرین | SYBR green qPCR mastermix

Name: مسترمیکس ریل تایم سایبرگرین
Brand: یکتا تجهیز آزما YTA
SKU: YT2551
Price: 12000000 IRR
Availability: InStock

مسترمیکس ریل تایم سایبرگرین (SYBR green qPCR mastermix) با غلظت 2x شرکت یکتا تجهیز آزما یک مستر میکس سریع و راحت برای انجام آزمایش های کمی qPCR بر پایه سایبرگرین هستند. رنگ سایبرگرین به صورت غیراختصاصی به DNA دو رشته ای متصل می شود. بنابراین یک سیگنال فلورسنت ارائه می دهد که مقدار محصولات dsDNA تولید شده را در طی PCR را منعکس می کند.

شدت فلورسانس SYBR پس از هر چرخه ، با تکثیر DNA افزایش می یابد و امکان اندازه گیری ماده ژنتیکی به صورت کمی فراهم می شود. این یک مسترمیکس تجاری آماده استفاده است و حاوی تمام اجزای لازم از جمله پلیمراز، رنگ سایبرگرین (SYBR green، dNTPs) و بافر هستند. فقط کافی است نمونه و پرایمرها را اضافه کنید.مسترمیکس ریل تایم سایبرگرین فاقد ROX بوده که امکان افزودن این رنگ مرجع به میزان دلخواه و بر اساس نیاز واکنش خود در دستگاههای qPCR که نیاز به حضور ROX دارند فراهم شده است.
مقاله کاربرد مسترمیکس سایبرگرین در برررسی های مولکولی

کاربردهای SYBR green qPCR mastermix:

تشخیص کمی براساس رنگ فلوروسنت متصل شونده به DNA
تکثیر نوکلئیک اسید و بررسی پروفایل بیانی
تکثیر DNA همه گونه ها از جمله DNA ژنومی، cDNA و DNA پلاسمیدی

شرایط نگهداری:

در دمای 20- نگهداری شود.
از انجماد و ذوب مکرر محصول خودداری کنید.

دستورالعمل ها

دستور العمل 2X SYBR Green qPCR Master MIx 1 ml

سوالات متداول

ژورنالهای علمی و پژوهشی

Published online 2022 Apr 27| Cell J | Read article

Down-Regulated Expression of Cystathionine β-Synthase and Cystathionine γ-Lyase in Varicocele, and Infertile Men: A Case-Control Study

mRNA expression analysis was accomplished via real-time polymerase chain reaction (PCR) by YTA SYBR Green qPCR MasterMix 2X (Yekta Tajhiz Azama, Iran) according to the manufacturer’s instruction in StepOnePlus Real-Time PCR System ... READ MORE

Published online 2019 Jun 18| 3 Biotech | Read article

**The concurrent effects of azurin and Mammaglobin-A genes in inhibition of breast cancer progression and immune system stimulation in cancerous BALB/c mice**

The reaction mixture in a 0.2-mL micro-tube at a final volume of 13 μL consisted of 6.5 μL of YTA 1× SYBR Green qPCR Mix (Yekta Tajhiz Azma, Tehran, Iran), 0.5 μL of each forward and reverse primer (2 μM), 1 μL cDNA specimen (50 ng), and 4.5 μL of distilled water...READ MORE

Published 2023 Mar| Journal List | Read article

Cannabidiol interacts with the FXR/Nrf2 pathway and changes the CB1/CB2 receptors ratio in gentamicin-induced kidney injury in rats

Then the cDNA synthesis was performed using a Reverse Transcriptase cDNA synthesis kit (Yekta Tajhiz Azma Co Iran), based on the protocol. Expression of the aforementioned genes was measured by Real-Time PCR using SYBR Green (Yekta Tajhiz Azma Co Iran). Experiments were performed in duplicates as follows...READ MORE

Published online 2021 Oct 30| Cell J | Read article

Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus

Resulting cDNA was subjected to quantitative real-time polymerase chain reaction (q-PCR) by using SYBR1 Green Mix (Yekta Tajhiz Azma, Iran) on a Light Cycler 96 System (Roche Diagnostics GmbH, Germany). The following conditions were used for q-PCR: initial heating for 10 minutes at 95˚C, 45 cycles of amplification...READ MORE

Published 2022 May| Avicenna J Phytomed | Read article

Effect of turmeric extract on bone healing in an experimental model of femoral bone fracture

For assessment of relative gene expression, the cDNA was amplified using SYBR green PCR master mix (YTA, Yekta Tajhiz Azma, Iran) for 45 cycles of 5 sec denaturation at 95°C, 20 sec annealing at 60°C, and 25 sec of amplification at 72°C using a...READ MORE

Published online 2023 May 12|Int J Reprod Biomed| Read article

Effect of dietary approaches to stop hypertension, and standard diets with and without curcumin on interleukin-1 alpha, 5-alpha reductase gene expressions, and androgenic and glycemic profile in polycystic ovary syndrome women undergoing in vitro fertilization treatment: A study protocol

IL-1α and 5α-reductase expression levels will be assessed by SYBR-green real-time PCR detection method using the kit protocol (Yekta Tajhiz Azma, IRAN), and the StepOne real-time PCR system (Applied Biosystems, Foster City, California, USA)...READ MORE

Published 2023 Feb|Int Journal List| Read article

Expression analysis elucidates the roles of Nicastrin, Notch4, and Hes1 in prognosis and endocrine-therapy resistance in ER-positive breast cancer patients

By employing specific primers (Table 2), the quantitative real-time polymerase chain reaction (qRT-PCR) was performed on synthesized cDNAs using the SYBR Green protocol. Based on the manufacturer’s instructions (YTA SYBR Green qPCR master mix 2X, Iran), the PCR experiment was conducted under the following condition... READ MORE

Published online 2023 2023 Sep| Int J Reprod Biomed| Read article

Crucial role of corticotropin-releasing hormone, corticotropin-releasing hormone -binding protein, mir-200c, and mir-181a in preterm delivery: A case-control study

qRT-PCR was performed using the SYBR Green PCR kit (Yekta Tajhiz Azma kit, Iran, www.yaktatajhiz.com) on the ABI thermal cycler detection system with designing primers (Table I), and expression levels were assessed using 2... READ MORE

Published online 2023 Jul-Sep | Avicenna J Med Biotechnol | Read article

Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias

We used the SYBR green (Yekta Tajhiz Azma, Iran) to determine PLAC1 expression levels. The specific primers were used as follows: PLAC1 sense, 5′-G TGAGCACAAAGCCACATTTC-3′ and antisense, 5′-GCAGCCAATCAGATAATGAACC-3′; GAPDH sense, 5′- GAAGGTGAAGGTCGGAGTC -3′ and antisense, 5′-GAAGATGGTGATGGGATTTC-3′ (Bioneer, Korea). The quantitative PCR...READ MORE

ارسال رایگان

مسترمیکس ریل تایم سایبرگرین

مسترمیکس ریل تایم سایبرگرین | SYBR green qPCR mastermix

کاربردهای SYBR green qPCR mastermix:

شرایط نگهداری:

دستورالعمل ها

دستور العمل 2X SYBR Green qPCR Master MIx 1 ml

سوالات متداول

ژورنالهای علمی و پژوهشی

Published online 2022 Apr 27| Cell J | Read article

Down-Regulated Expression of Cystathionine β-Synthase and Cystathionine γ-Lyase in Varicocele, and Infertile Men: A Case-Control Study

mRNA expression analysis was accomplished via real-time polymerase chain reaction (PCR) by YTA SYBR Green qPCR MasterMix 2X (Yekta Tajhiz Azama, Iran) according to the manufacturer’s instruction in StepOnePlus Real-Time PCR System ... READ MORE

Published online 2019 Jun 18| 3 Biotech | Read article

**The concurrent effects of azurin and Mammaglobin-A genes in inhibition of breast cancer progression and immune system stimulation in cancerous BALB/c mice**

The reaction mixture in a 0.2-mL micro-tube at a final volume of 13 μL consisted of 6.5 μL of YTA 1× SYBR Green qPCR Mix (Yekta Tajhiz Azma, Tehran, Iran), 0.5 μL of each forward and reverse primer (2 μM), 1 μL cDNA specimen (50 ng), and 4.5 μL of distilled water...READ MORE

Published 2023 Mar| Journal List | Read article

Cannabidiol interacts with the FXR/Nrf2 pathway and changes the CB1/CB2 receptors ratio in gentamicin-induced kidney injury in rats

Published online 2021 Oct 30| Cell J | Read article

Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus

Published 2022 May| Avicenna J Phytomed | Read article

Effect of turmeric extract on bone healing in an experimental model of femoral bone fracture

For assessment of relative gene expression, the cDNA was amplified using SYBR green PCR master mix (YTA, Yekta Tajhiz Azma, Iran) for 45 cycles of 5 sec denaturation at 95°C, 20 sec annealing at 60°C, and 25 sec of amplification at 72°C using a...READ MORE

Published online 2023 May 12|Int J Reprod Biomed| Read article

Effect of dietary approaches to stop hypertension, and standard diets with and without curcumin on interleukin-1 alpha, 5-alpha reductase gene expressions, and androgenic and glycemic profile in polycystic ovary syndrome women undergoing in vitro fertilization treatment: A study protocol

IL-1α and 5α-reductase expression levels will be assessed by SYBR-green real-time PCR detection method using the kit protocol (Yekta Tajhiz Azma, IRAN), and the StepOne real-time PCR system (Applied Biosystems, Foster City, California, USA)...READ MORE

Published 2023 Feb|Int Journal List| Read article

Expression analysis elucidates the roles of Nicastrin, Notch4, and Hes1 in prognosis and endocrine-therapy resistance in ER-positive breast cancer patients

Published online 2023 2023 Sep| Int J Reprod Biomed| Read article

Crucial role of corticotropin-releasing hormone, corticotropin-releasing hormone -binding protein, mir-200c, and mir-181a in preterm delivery: A case-control study

qRT-PCR was performed using the SYBR Green PCR kit (Yekta Tajhiz Azma kit, Iran, www.yaktatajhiz.com) on the ABI thermal cycler detection system with designing primers (Table I), and expression levels were assessed using 2... READ MORE

Published online 2023 Jul-Sep | Avicenna J Med Biotechnol | Read article

Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias

مستر میکس ریل تایم اسمارت گرین | Smart Green qPCR (real time) mastermix

مسترمیکس Hot start primer block

آنزیم Hot Start Taq DNA Polymerase

مسترمیکس مالتی پلکس PCR ‌با UDG

آنزیم MMLV

کیت سنتز سی دی اِن آ | cDNA synthesis Kit

آنزیم Fast Pfu DNA پلی مراز | Fast pfu DNA polymerase

آنزیم DNase I

مسترمیکس ریل تایم سایبرگرین

مسترمیکس ریل تایم سایبرگرین | SYBR green qPCR mastermix

کاربردهای SYBR green qPCR mastermix:

شرایط نگهداری:

دستورالعمل ها

دستور العمل 2X SYBR Green qPCR Master MIx 1 ml

سوالات متداول

ژورنالهای علمی و پژوهشی

Published online 2022 Apr 27| Cell J | Read article

Down-Regulated Expression of Cystathionine β-Synthase and Cystathionine γ-Lyase in Varicocele, and Infertile Men: A Case-Control Study

mRNA expression analysis was accomplished via real-time polymerase chain reaction (PCR) by YTA SYBR Green qPCR MasterMix 2X (Yekta Tajhiz Azama, Iran) according to the manufacturer’s instruction in StepOnePlus Real-Time PCR System ... READ MORE

Published online 2019 Jun 18| 3 Biotech | Read article

The concurrent effects of azurin and Mammaglobin-A genes in inhibition of breast cancer progression and immune system stimulation in cancerous BALB/c mice

The reaction mixture in a 0.2-mL micro-tube at a final volume of 13 μL consisted of 6.5 μL of YTA 1× SYBR Green qPCR Mix (Yekta Tajhiz Azma, Tehran, Iran), 0.5 μL of each forward and reverse primer (2 μM), 1 μL cDNA specimen (50 ng), and 4.5 μL of distilled water...READ MORE

Published 2023 Mar| Journal List | Read article

Cannabidiol interacts with the FXR/Nrf2 pathway and changes the CB1/CB2 receptors ratio in gentamicin-induced kidney injury in rats

Published online 2021 Oct 30| Cell J | Read article

Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus

Published 2022 May| Avicenna J Phytomed | Read article

Effect of turmeric extract on bone healing in an experimental model of femoral bone fracture

For assessment of relative gene expression, the cDNA was amplified using SYBR green PCR master mix (YTA, Yekta Tajhiz Azma, Iran) for 45 cycles of 5 sec denaturation at 95°C, 20 sec annealing at 60°C, and 25 sec of amplification at 72°C using a...READ MORE

Published online 2023 May 12|Int J Reprod Biomed| Read article

Effect of dietary approaches to stop hypertension, and standard diets with and without curcumin on interleukin-1 alpha, 5-alpha reductase gene expressions, and androgenic and glycemic profile in polycystic ovary syndrome women undergoing in vitro fertilization treatment: A study protocol

IL-1α and 5α-reductase expression levels will be assessed by SYBR-green real-time PCR detection method using the kit protocol (Yekta Tajhiz Azma, IRAN), and the StepOne real-time PCR system (Applied Biosystems, Foster City, California, USA)...READ MORE

Published 2023 Feb|Int Journal List| Read article

Expression analysis elucidates the roles of Nicastrin, Notch4, and Hes1 in prognosis and endocrine-therapy resistance in ER-positive breast cancer patients

Published online 2023 2023 Sep| Int J Reprod Biomed| Read article

Crucial role of corticotropin-releasing hormone, corticotropin-releasing hormone -binding protein, mir-200c, and mir-181a in preterm delivery: A case-control study

qRT-PCR was performed using the SYBR Green PCR kit (Yekta Tajhiz Azma kit, Iran, www.yaktatajhiz.com) on the ABI thermal cycler detection system with designing primers (Table I), and expression levels were assessed using 2... READ MORE

Published online 2023 Jul-Sep | Avicenna J Med Biotechnol | Read article

Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias

محصولات مرتبط

**The concurrent effects of azurin and Mammaglobin-A genes in inhibition of breast cancer progression and immune system stimulation in cancerous BALB/c mice**