Ribonuclease A (RNase A)

Extracted from bovine pancreas for RNA separation, especially when isolating RNAfree DNA. RNase A is an endoribonuclease that attacks at the phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing disulfide bridges. Activators of RNase A include potassium and sodium salts.

Applications:

• Removal of RNA from recombinant protein preparations
• Ribonuclease protection assays
• Removal of non-hybridized regions of RNA:DNA hybrids
• Mapping single-base mutations in DNA or RNA
• removal of RNA from preparations of plasmid DNA

storage:

• store at –20 °C