Whole Blood RNA Mini Kit

Features:

High Purity: OD260/280: 1.9-2.1
Safe Use: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to, and disposal of hazardous materials.
No risk or enzymatic inhibition by phenol/chloroform
RNA is suitable for downstream applications

توضیحات

Whole Blood RNA Mini Kit is designed for rapid and efficient isolation of total RNA from frozen and fresh whole blood samples. The kit uses a chaotropic salt-based formula for cell lysis, RNase inactivation and RNA binding (>200 nt, e.g., 18S, 28S RNA, pri-miRNA) to silica membrane. The optional on-column DNase I digestion technique offers a convenient DNA removal method. The high-purity RNA is eluted from the membrane by using a low-ionic-strength buffer. This extracted total RNA is ready-to-use for downstream applications, such as real-time RT-PCR, cDNA synthesis, Northern blotting, primer extension, miRNA selection, etc.

Sample types

whole blood

Specification

Format: mini spin column (RNA Binding Column)
Principle: silica-membrane technology/chaotropic salt binding
Sample size: 200~400 µl of whole blood
Size of isolated RNA: >200 nucleotides
Typical RNA yield: 5~7 µg of whole blood
Operation time: ≤50 mins
Binding capacity: ≤100 µg RNA/Column
Column applicability: centrifugation and vacuum
Minimum elution volume: 30 µ

Documents

protocol of Whole Blood RNA Mini Kit

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