The Genomic DNA Mini Kit (Blood/Cultured Cell) provides an efficient silica membrane-based method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood, plasma, serum and other body fluids. No phenol–chloroform extraction is required. DNA binds exactly to the silica-gel membrane while contaminants pass through. Also PCR inhibitors, such as divalent cations and proteins, are totally removed in two effective wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit.The purified, high-quality DNA is then ready to use in a wide variety of demanding downstream applications, such as PCR.
You can also refer to Tissue genomic DNA extraction mini kit.
Sample Types :
DNA Blood Kits provide proven technology for the purification of DNA from a variety of samples:
- Fresh and frozen whole blood or buffy coat
- Plasma or serum
- Bone marrow
- Body fluids
- Cultured cells
Technology: spin column (silica membrane)
Sample: up to 200 µl whole blood, serum, plasma, body fluids , lymphocytes up to 5 x10 ⁶cultured cells
Operation time: < 30 min
Binding capacity: up to 60 µg/ column
DNA Yield: 4~8 µg/ 200 µl whole blood
This kit is considered for a variety of sample sizes from 200 μl fresh or frozen human whole blood up to 5 x 10 for cultured cells . spin columns can be easily managed in a centrifuge or on vacuum manifolds.