cDNA Synthesis Kit
Features :
- Easy to use
- High sensitivity reverse transcription from low abundance template
- Broad linear dynamic range of total input RNA (0.1ng – 5μg) with a highly efficient MMLV reverse transcriptase
- Complete cDNA synthesis and qPCR on the same day
- Primers are lyophilized
- Contains both Oligo(dT)18 primer and Random Hexamer
cDNA synthesis kit offer whole components you need for cDNA synthesize include RNase inhibitor, Primer,dNTP mixture, Buffer and M-MuLV RT enzyme . M-MuLV is a reverse transcriptase enzyme with optimum activity at 37C and no RNase H activity.for greater application flexibility we provide both random hexamer and Oligo dt primers.This kit provides superior reagents that ensure efficient first-strand cDNA synthesis from mRNA or total RNA templates.
Applications :
- First-strand cDNA synthesis for RT-PCR and qPCR
- Gene expression profiling
- Reagents can be used for miRNA synthesis
Storage:
- store at -20ᴼC
Details
protocol of cDNA Synthesis Kit → download
Scientific and research journals
Published online 2023 Apr 27 | Adv Biomed Res | Read article
Evaluation of Rap1GAP and EPAC1 Gene Expression in Endometriosis Disease
. All wome participating in this study were from the Iranian population living in Yazd. To maintain mRNA stability, the tissues were kept in RNA stabilizer solution (RNA later, Yekta Tajhiz Azma, Iran) at -80°C. The inclusion criteria....READ MORE
Published online 2023 Mar 21 | BMC Microbiol | Read article
Evaluation of the mTORC activity in the presence of Toxoplasma gondii and azathioprine in human monocyte cell line
Total RNA was extracted from THP-1 cell line in each tested well using total RNA extraction kit (Yekta Tajhiz Azma, Tehran, Iran) in accordance with the manufacturer’s protocol . Extracted RNA was purified by DNase (Thermo Fisher Scientific) treatment, and its concentration was calculated by ...READ MORE
Published online 2023 Mar| Iran J Basic Med Sci | Read article
Cannabidiol interacts with the FXR/Nrf2 pathway and changes the CB1/CB2 receptors ratio in gentamicin-induced kidney injury in rats
The RNA then was extracted using Trizol (Yekta Tajhiz Azma Co Iran) based on the manufacturer’s instructions. Then the cDNA synthesis was performed using a Reverse Transcriptase cDNA synthesis kit (Yekta Tajhiz Azma Co Iran), based on the protocol. Expression of the aforementioned genes was measured by Real-Time PCR ...READ MORE
Published online 2023 Mar 20| Springer Nature | Read article
Oral vaccination with novel Lactococcus lactis mucosal live vector-secreting Brucella lumazine synthase (BLS) protein induces humoral and cellular immune protection against Brucella abortus
instructions for RNA extraction and cDNA synthesis (Yekta Tajhiz, Iran). The GAPDH gene was utilized as an internal control during real-time PCR using the YTA SYBR Green master mix (Yekta Tajhiz, Iran). For real Time-PCR, a reaction volume ...READ MORE
Published online 2023| Iran J Basic Med Sci | Read article
Effects of exosomes of mesenchymal stem cells on cholesterol-induced hepatic fibrogenesis
Total RNA wasTotal RNA was extracted using an RNA Isolation Kit (Yekta Tajhiz Azma, Iran) according to the manufacturer’s instructions. The complementary DNA (cDNA) was synthesized by using a cDNA synthesis kit (Yekta Tajhiz Azma, Iran) READ MORE
Published online 2023 Aug 2| J Cell Mol Med | Read article
Characterisation of extracellular vesicles isolated from hydatid cyst fluid and evaluation of immunomodulatory effects on human monocytes
Total RNA was extracted after 3 and 24 h by total RNA extraction kit (Yekta Tajhiz Azma), according to the manufacturer's protocol. DNase (Thermo Fisher Scientific™) treatment was performed to remove the probable residual DNA and to improve the quality of the extracted RNA, according to the manufacturer's protocol. The RNA solution was finally collected and stored at −70 ° C until cDNA synthesis. Prior to cDNA synthesis, the concentration of purified RNA...READ MORE
Published online 2023 Jun 26| Scientific Reports | Read article
Integrated bioinformatics and wet-lab analysis revealed cell adhesion prominent genes CDC42, TAGLN and GSN as prognostic biomarkers in colonic-polyp lesions
Total RNAs were extracted from obtained samples (Yekta Tajhiz Azma kit, Cat No. YT9065, Teheran, Iran). The RNAs concentration was measured by Nanodrop (NanoDrop Technologies, Wilmington, DE, USA) for acceptable quantification and qualification. RNAs were converted to cDNA by Retro transcriptase (RT) reaction (Yekta Tajhiz Azma kit, Cat No. YT9065, Teheran, Iran) after...READ MORE
Published online 2023 Jun 5| Vet Med Sci | Read article
Brucella abortus antigen omp25 vaccines: Development and targeting based on Lactococcus lactis
The PCR reaction was conducted to monitor omp25 using specific primers (Table 1). A 20‐mL PCR reaction contains 2 mL of ×10 PCR buffer (Yekta Tajhiz Azma, Iran), 2 mM MgCl2, 200 μM dNTPs (Yekta Tajhiz Azma, Iran), 10 pmol of each primer (CinnaGen, Iran), 100 ng of plasmid DNA and 1 unit of Taq DNA polymerase enzyme (Yekta Tajhiz Azma, Iran). The PCR ...READ MORE
Published online 2023 Feb 2| Elsevier - PMC COVID-19 Collection | Read article
Evaluation of Tcell exhaustion based on the expression of EOMES, Tbet and co-inhibitory receptors in severe and non-severe covid-19 patients
Initially, total RNA extraction of the blood sample collected from all participants was performed using the total RNA extraction kit (Yekta Tajhiz Azma kit, Teheran, Iran). A Nanodrop spectrophotometer (Plat reader epoch 2) was used to...READ MORE
Published online 2023 Jul 29| Bioimpacts | Read article
Comparison the therapeutic effects of bone marrow CD144+ endothelial cells and CD146+ mesenchymal stem cells in POF rats
Subsequently, defined concentrations of RNA were reverse-transcribed using a cDNA synthesis kit (Cat No: YT4500, Yekta Tajhiz Azma). Primers targeting target genes were designed using Primer-Blast online software...READ MORE
Published online 2023 Nov 8| Scientific Reports| Read article
Using machine learning approach for screening metastatic biomarkers in colorectal cancer and predictive modeling with experimental validation
We extracted total cellular RNA from both fresh frozen and paraffin-embedded tissues using the RNX kit (RNX, Cina Gene Company, Iran). Then Yekta Tajhiz cDNA Synthesis Kit (Yekta Tajhiz Azama, Iran) was used to convert the extracted RNA into cDNA...READ MORE
Published online 2023 Jan 11| Cell Prolif | Read article
The beneficial effects of chick embryo extract preconditioning on hair follicle stem cells: A promising strategy to generate Schwann cells
Total RNA was extracted from cells grown in 6‐well plates at ~90% confluency, using YTzol (Cat. No: YT9063; Yekta Tajhiz Azma, Iran) reagent by chloroform extraction and ethanol precipitation, according to the manufacturer's instructions, followed by...READ MORE
Published online 2023 Apr 3| BMC Complement Med Ther| Read article
Chamomile and Urtica dioica extracts improve immunological and histological alterations associated with polycystic ovarian syndrome in DHEA -induced mice
The adipose tissue surrounding the right ovary and uterus was separated and homogenized in the RNA Lysis buffer. Then, after extracting total RNA and synthesizing cDNA(Yekta Tajhiz, Iran), the primers of the target genes and a housekeeping gene (GAPDH) were designed using Allele ID 6 software (Premier Bio soft, USA) and aligned on BLAST website (Table 1). Real-time PCRs were...READ MORE
Published online 2023 Feb 14| Cell J| Read article
The Immunomodulatory Aspect of Quercetin Penta Acetate on Th17 Cells Proliferation and Gene Expression in Multiple Sclerosis
First, total RNA was isolated from the cultured PBMCs using an extraction kit (Yekta tajhiz azma Co. Iran) according to the manufacture instructions. The purity of isolated RNA was determined using a spectrophotometer. The first strand cDNA (Yekta tajhiz azma Co. Iran) ...READ MORE
Published online 2023 Jun 13.| Arch Med Sci| Read article
An in vitro investigation of the effects of urolithins A and B on low-density lipoprotein uptake and its regulatory genes
a cDNA synthesis kit (YT4500, Yekta Tajhiz Azma, Iran), DNase/RNase free water (CH8161, Sinaclon, Iran), and a QPCR master mix kit (BN-0011.17.4, Tehran, Iran), were used for tests...READ MORE
Published online 2023 Nov 8|Sci Rep| Read article
Nuclear overexpression of DNA damage-inducible transcript 4 (DDIT4) is associated with aggressive tumor behavior in patients with pancreatic tumors
extracted RNAs were measured in quantity and quality by Nanodrop (ThermoFisher Scientific, USA). Then RNA was reverse transcript into cDNA using the cDNA Synthesis Kit (Yekta Tajhiz Azma, YT4500, Iran).... READ MORE
Published online 2023 Sep 30|Prev Nutr Food Sci| Read article
Influence of 8 Weeks of Tabata High-Intensity Interval Training and Nanocurcumin Supplementation on Inflammation and Cardiorespiratory Health among Overweight Elderly Women
Total RNA, including lncRNA-MIAT and NLRP3 inflammasome coding, was extracted using a commercial kit (Yekta Tajhiz Co.), according to the manufacturer’s instructions... READ MORE
Published online 2023 Mar 1|Prev Nutr Food Sci| Read article
A1 adenosine receptor antagonist induces cell apoptosis in KYSE-30 and YM-1 esophageal cancer cell lines
Total RNA was extracted using the RNX plus reagent (Sinaclon, Iran) following the manufacturer’s instructions. The RevertAid First Strand cDNA Synthesis Kit (Yekta Tajiz, Iran) was used to... READ MORE
Published online 2023 Nov 21|Front Bioeng Biotechnol| Read article
Enhancing productivity of Chinese hamster ovary (CHO) cells: synergistic strategies combining low-temperature culture and mTORC1 signaling engineering
The total RNA was extracted from transfected cells using a Trizol reagent (Bio Basic, Canada) according to the manufacturer’s protocol. cDNA synthesis was carried out using the Yekta Tajhiz Azma cDNA Synthesis Kit (YT4500; Tehran, Iran). The real-time RT-qPCR analysis READ MORE
Published online 2023 |Iran J Basic Med Sci| Read article
Effect of a combined program of running exercise and environmental enrichment on memory function and neurogenesis markers in amyloid-beta-induced Alzheimer-like model
. First-strand cDNA was synthesized from 1000 ng of RNA using a cDNA Synthesis Kit (Yekta-Tajhiz; Cat: Yt4500), following the manufacturer’s instructions. qRT-PCR amplification was performed using the CFX 96 Real-Time System (Roche Applied Science, USA) and Syber Green dye... READ MORE
Published on 2023 |Int J Mol Cell Med| Read article
Amyloid Beta Alters the Expression of microRNAs Regulating HMGCR and ABCA1 Genes in Astrocytes of C57BL/6J Mice
For cDNA synthesis, the Yekta Tajhiz Azma cDNA kit was used with random hexamer and oligo-dT primers. In addition, to synthesize cDNA from miRNA, the BONmiR 1 st-strand cDNA synthesis kit (Bon Yakhteh Technology Research Center, Iran) was used... READ MORE
Published online 2023 Oct 21 |Int J Mol Cell Med| Read article
Metabolic and Transcriptomic Approaches of Chitosan and Water Stress on Polyphenolic and Terpenoid Components and Gene Expression in Salvia abrotanoides (Karl.) and S. yangii
To obtain the cDNA, the total RNA (1 μg) was reverse transcribed to cDNA using a cDNA synthesis kit (Yekta Tajhiz Azma, St. Rashid, Tehran, Iran), according to... READ MORE
Published online 2023 Aug 28 |Heliyon| Read article
Ameliorating inflammation in an in vitro model by screening the anti-inflammatory and immunomodulatory roles of putative probiotics in inflammatory bowel disease
An RNA extraction kit (Roche, Germany) and the cDNA synthesis kit (Yekta Tajhiz, Iran) were used according to the manufacturer's instructions. The online website Primer Bank... READ MORE
Published online 2023 Sep 21|BMC Complement Med Ther| Read article
Investigation of the anti-inflammatory effects of native potential probiotics as supplementary therapeutic agents in an in-vitro model of inflammation
. The quantity and quality of the purified RNA were determined by a NanoDrop1000 UV-Vis Spectrophotometer (measuring the absorbance at 260/280 nm). The cDNA template was synthesized with the cDNA synthesis kit (Yekta Tajhiz, Iran) according to the manufacturer’s instructions....READ MORE
Specification :
- store at -20ᴼC
- Primers are lyophilized
- Contains both Oligo(dT)18 primer and Random Hexamer
- It can be used with either sequence-specific primers, poly(dT)18primers, or random Hexamer
Applications :
- PCR, see Protocol for First-strand cDNA Synthesis;
- Second strand cDNA synthesis.
- DNA labeling.
- Real-time PCR;
- Analysis of RNA by primer extension.
For greater application flexibility, hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, for the synthesis of cDNA from only poly(A) tailed mRNA, are included.
You can also refer to MMLV reverse transcriptase .
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