Whole Blood RNA Mini Kit is designed for rapid and efficient isolation of total RNA from frozen and fresh whole blood samples. The kit uses a chaotropic salt-based formula for cell lysis, RNase inactivation and RNA binding (>200 nt, e.g., 18S, 28S RNA, pri-miRNA) to silica membrane. The optional on-column DNase I digestion technique offers a convenient DNA removal method. The high-purity RNA is eluted from the membrane by using a low-ionic-strength buffer. This extracted total RNA is ready-to-use for downstream applications, such as real-time RT-PCR, cDNA synthesis, Northern blotting, primer extension, miRNA selection, etc.
Sample types
whole blood
Specification
Format: mini spin column (RNA Binding Column)
Principle: silica-membrane technology/chaotropic salt binding
Sample size: 200~400 µl of whole blood
Size of isolated RNA: >200 nucleotides
Typical RNA yield: 5~7 µg of whole blood
Operation time: ≤50 mins
Binding capacity: ≤100 µg RNA/Column
Column applicability: centrifugation and vacuum
Minimum elution volume: 30 µ