2× PCR mastermix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. The mix retains all features of Taq DNA Polymerase. Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75 ° C). It has 5′ to 3′ polymerase activity but lacks of 3′ to 5′ exonuclease activity that results in a 3′-dA overhangs PCR product.
Applications:
• High throughput PCR
• Routine PCR with high reproducibility
• Generation of PCR products for TA cloning
