YTA Loading Dye

Features :

  • Two-color tracking of DNA migration during electrophoresis.
  • No DNA masking during gel exposure to UV light.
  • EDTA binds divalent metal ions and inhibits metal dependent nucleases.
Description

YTA Loading Dye (6×) is a mixer loading buffer with two tracking dyes for agarose and non-denaturing polyacrylamide gel electrophoresis. It contains a compound to render your samples denser than the running buffer, so that the samples sink in the well when loading; and a dye that migrates during electrophoresis at a similar rate to DNA. Bromophenol blue and xylene cyanol are used in this mixture as the standard tracking dyes for electrophoresis.

Documents

Documents

Download the protocol

Details

 

Specification:

  • assayed for absence of endonuclease, exonuclease and no RNase activity.
  • Add 1 volume of 6× Gel Loading Dye to 5 volumes of DNA sample.
  • Contents : Tris-HCl (pH 7.6), Bromophenol Blue, Xylene Cyanol, Glycerol and EDTA.

Storage :

  • Store at RT or at 4˚C for up to 12 months. For longer periods, store at -20˚C.