YTzol Pure RNA solution is a ready-to-use reagent, considered to extract high quality total RNA (plus DNA and proteins) from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, during one hour. YTzol Pure RNA solution is a monophasic solution of phenol , guanidine isothiocyanate , and other exclusive mechanisms which simplify the isolation of a range of RNA types of large or small molecular size. YTzol Pure RNA solution keeps the integrity of the RNA due to highly active inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. YTzol Pure RNA solution permits for concurrent processing of a large number of samples . YTzol Pure RNA solution allows to complete sequential precipitation of RNA, DNA, and proteins from a single sample .
After homogenizing the sample with YTzol Pure RNA solution, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interphase, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.
• Isolated RNA can be used in RT-PCR, Northern Blot analysis, Dot Blot hybridization, poly(A)+ selection, in vitro translation, RNase protection assay, and molecular cloning.
• Isolated DNA can be used in PCR, Restriction Enzyme digestion, and Southern Blots.
• Isolated protein can be used for Western Blots, recovery of some enzymatic activity, and some immunoprecipitation.
YTzol Pure RNA solution can also be used with Phasemaker Tubes to isolate RNA.
Sample Types :
Tissues(human, animal, plant)
Cells grown in monolayer
Cells grown in suspension from animal, plant, yeast or cells of bacterial origin
