YTA SYBR Green qPCR Mastermix
Features:
- Ready to use mixture
- Containing Hot-start technology
- High sensitivity and specificity
- Compatible with many Real-time systems which not require ROX reference dye
YTA SYBR Green qPCR Mix is considered for high-performance, high-throughput real-time PCR. The kit covers Taq DNA Polymerase engineered through a process of molecular evolution. The result is a unique polymerase that exactly intended for qPCR using SYBR Green I chemistry dye.
YTA SYBR Green qPCR MasterMix is a convenient premix of the components (except primers and DNA template) that essential to perform real-time polymerase chain reaction (PCR) using SYBR Green I dye with enhanced sensitivity and specificity. The SYBR Green I dye binds to double-stranded DNA (dsDNA), therefore providing a fluorescent signal that reflects the amount of dsDNA products generated during PCR.
Applications
- Gene expression analysis
- Low-copy gene detection
- Microarray validation
- Gene knockdown validation
Storage :
This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.
Documents
Download the protocol
Scientific and research journals
Published online 2022 Apr 27| Cell J | Read article
Down-Regulated Expression of Cystathionine β-Synthase and Cystathionine γ-Lyase in Varicocele, and Infertile Men: A Case-Control Study
mRNA expression analysis was accomplished via real-time polymerase chain reaction (PCR) by YTA SYBR Green qPCR MasterMix 2X (Yekta Tajhiz Azama, Iran) according to the manufacturer’s instruction in StepOnePlus Real-Time PCR System ... READ MORE
Published online 2019 Jun 18| 3 Biotech | Read article
The concurrent effects of azurin and Mammaglobin-A genes in inhibition of breast cancer progression and immune system stimulation in cancerous BALB/c mice
The reaction mixture in a 0.2-mL micro-tube at a final volume of 13 μL consisted of 6.5 μL of YTA 1× SYBR Green qPCR Mix (Yekta Tajhiz Azma, Tehran, Iran), 0.5 μL of each forward and reverse primer (2 μM), 1 μL cDNA specimen (50 ng), and 4.5 μL of distilled water...READ MORE
Published 2023 Mar| Journal List | Read article
Cannabidiol interacts with the FXR/Nrf2 pathway and changes the CB1/CB2 receptors ratio in gentamicin-induced kidney injury in rats
Then the cDNA synthesis was performed using a Reverse Transcriptase cDNA synthesis kit (Yekta Tajhiz Azma Co Iran), based on the protocol. Expression of the aforementioned genes was measured by Real-Time PCR using SYBR Green (Yekta Tajhiz Azma Co Iran). Experiments were performed in duplicates as follows...READ MORE
Published online 2021 Oct 30| Cell J | Read article
Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus
Resulting cDNA was subjected to quantitative real-time polymerase chain reaction (q-PCR) by using SYBR1 Green Mix (Yekta Tajhiz Azma, Iran) on a Light Cycler 96 System (Roche Diagnostics GmbH, Germany). The following conditions were used for q-PCR: initial heating for 10 minutes at 95˚C, 45 cycles of amplification...READ MORE
Published 2022 May| Avicenna J Phytomed | Read article
Effect of turmeric extract on bone healing in an experimental model of femoral bone fracture
For assessment of relative gene expression, the cDNA was amplified using SYBR green PCR master mix (YTA, Yekta Tajhiz Azma, Iran) for 45 cycles of 5 sec denaturation at 95°C, 20 sec annealing at 60°C, and 25 sec of amplification at 72°C using a...READ MORE
Published online 2023 May 12|Int J Reprod Biomed| Read article
Effect of dietary approaches to stop hypertension, and standard diets with and without curcumin on interleukin-1 alpha, 5-alpha reductase gene expressions, and androgenic and glycemic profile in polycystic ovary syndrome women undergoing in vitro fertilization treatment: A study protocol
IL-1α and 5α-reductase expression levels will be assessed by SYBR-green real-time PCR detection method using the kit protocol (Yekta Tajhiz Azma, IRAN), and the StepOne real-time PCR system (Applied Biosystems, Foster City, California, USA)...READ MORE
Published 2023 Feb|Int Journal List| Read article
Expression analysis elucidates the roles of Nicastrin, Notch4, and Hes1 in prognosis and endocrine-therapy resistance in ER-positive breast cancer patients
By employing specific primers (Table 2), the quantitative real-time polymerase chain reaction (qRT-PCR) was performed on synthesized cDNAs using the SYBR Green protocol. Based on the manufacturer’s instructions (YTA SYBR Green qPCR master mix 2X, Iran), the PCR experiment was conducted under the following condition... READ MORE
Published online 2023 2023 Sep| Int J Reprod Biomed| Read article
Crucial role of corticotropin-releasing hormone, corticotropin-releasing hormone -binding protein, mir-200c, and mir-181a in preterm delivery: A case-control study
qRT-PCR was performed using the SYBR Green PCR kit (Yekta Tajhiz Azma kit, Iran, www.yaktatajhiz.com) on the ABI thermal cycler detection system with designing primers (Table I), and expression levels were assessed using 2... READ MORE
Published online 2023 Jul-Sep | Avicenna J Med Biotechnol | Read article
Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias
We used the SYBR green (Yekta Tajhiz Azma, Iran) to determine PLAC1 expression levels. The specific primers were used as follows: PLAC1 sense, 5′-G TGAGCACAAAGCCACATTTC-3′ and antisense, 5′-GCAGCCAATCAGATAATGAACC-3′; GAPDH sense, 5′- GAAGGTGAAGGTCGGAGTC -3′ and antisense, 5′-GAAGATGGTGATGGGATTTC-3′ (Bioneer, Korea). The quantitative PCR...READ MORE
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