Bacteriophage T7 RNA Polymerase is a DNA dependent RNA polymerase that is routinely used to produce RNA sequences from DNA templates . The enzyme catalyzes the 5’→3’ synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from the promoter with extremely high specificity for the T7 promoter. T7 RNA Polymerase also can use modified nucleotides(e.g., biotin-, digoxigenin-, fluorescein-labeled nucleotides) as substrates for RNA synthesis.
Applications:
- In vitro Synthesis of RNA
- Synthesis of highly radiolabeled RNA probes
- Synthesis of RNA as hybridization probes
- RNA synthesis for in vitro translation and RNA splicing studies
- In studies of RNA secondary structure and RNA-protein interactions
