T4 DNA ligase
Features:
- Joining blunt end and cohesive end
- Recombinant enzyme isolated from E.coli
- Applicable for use in high-complexity library cloning
Description
YTA-T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5′ phosphate group of the donor and the 3′ hydroxyl group of the ligand to form a 3′ → 5′ phosphodiester bond in either a cohesive-ended or blunt-ended configuration. the substrate of this enzyme is double stranded DNA and will not join single stranded nucleic acids.
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