passive Reference Dye

Passive Reference Dye (ROX) is used as an internal reference dye when performing qPCR using compatible systems. This dye helps minimize fluorescence fluctuations between wells in a qPCR instrument by providing a consistent level of fluorescence in each well throughout the reaction. ROX does not affect the outcome of the qPCR reaction.

The required concentration of ROX in the mastermix depends on the Real-Time PCR instrument being used. Some systems require a high ROX concentration, while others may not need ROX at all for fluorescence normalization. However, in general, having a passive reference standard can be helpful to correct for pipetting variability and reaction setup errors.

Passive: This means the dye does not participate in or influence the reaction. It is used solely for signal adjustment and calibration, without impacting the reaction outcome.

Reference: Indicates that the dye serves as a stable baseline used for comparison throughout the experiment or measurement.

Dye: Dyes are substances that emit light at a specific wavelength when excited by light of another wavelength. This property is useful for signal detection and data analysis.