miRNA Isolation Kit is designed for purification of microRNAs (miRNAs) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNAs allows research into biological significant pathways for gene regulation. The standard protocols for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNAs and other small RNA. In addition, removal of the predominant larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of different sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.
Sample Types :
- Tissue
- cultured cells
- serum
Specification :
- Principle: mini spin column (silica matrix)
- Sample size: up to 1 x10 cultured cells
- up to 100 mg tissue
- Operation time: ~ 30 minutes
- Column applicability: centrifugation and vacuum
