Dithiothreitol DTT
DTT is a small-molecule redox reagent which used as reducing agent in molecular biology. It widely used in biochemistry works, in sample preparation and polyacrylamide electrophoresis. DTT forms a stable six-membered ring with an internal disulfide bond once oxidized. The use of DTT was popularized by William Wallace Cleland who first published on its reducing potential in 1964. Therefor dithiothreitol being referred to as Cleland’s reagent
Application
frequently used to reduce the disulfide bonds of proteins
can be used as an oxidizing agent
widely used in biochemistry works
can be used in molecular process
