Dithiothreitol DTT

 Dithiothreitol DTT

DTT is a small-molecule redox reagent which used as reducing agent in molecular biology. It widely used in biochemistry works, in sample preparation and polyacrylamide electrophoresis. DTT forms a stable six-membered ring with an internal disulfide bond once oxidized. The use of DTT was popularized by William Wallace Cleland who first published on its reducing potential in 1964.  Therefor dithiothreitol being referred to as Cleland’s reagent

Application

frequently used to reduce the disulfide bonds of proteins

can be used as an oxidizing agent

widely used in biochemistry works

can be used in molecular process