MMLV Thermostable
Features:
- Optimum reaction temperature is 37°C to 55°C
- Highest reaction temperature is 60°C
- Produces higher yield of cDNA
- Synthesis longer cDNA up to 10kb
Description
Thermostable Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an engineered version of M-MLV reverse transcriptase with reduced RNase H activity increased thermal stability. This enzyme have a polymerase feature and uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. M-MLV thermostable enzyme is an ideal choice for higher cDNA yield from low starting amounts of RNA. The broad range of temperature up to 60°C delivers reverse transcription at your temperature of choice. The enzyme can generate first-strand cDNA up to 10 kb.
Applications:
- RT-LAMP
- First-strand cDNA Synthesis
- One-step qRT-PCR
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