M-MLV (H-) Reverse Transcriptase
Features :
- Provides optimal conditions to prepare full-length cDNA
- High cDNA yield
- Contains no RNase H activity
- Optimal activity at 37°C
Description
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 5 kb.
Applications :
- The first-strand cDNA synthesis; RT-PCR
Storage :
- store at -20ᴼC
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