Ficoll 400

Ficoll 400 is a High molecular weight sucrose-polymers which formed by copolymerization of sucrose with epichlorohydrin.  Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, it is very hydrophilic and extremely water-soluble. The most common application is as a density gradient medium for the separation and isolation of eukaryotic cells, organelles and bacterial cells.

Application:

• Proven medium for density gradient centrifugation
• density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells
• separating cells that are sensitive to centrifugation
• separating cells of similar density but different size (under conditions of sedimentation at unit gravity)
• can be used as the raw material for preparation of Ficoll-Paque gradient
• Used in other applications such as hybridization, cryopreservation, hapten carrier and electrophoresis