Deoxyribonuclease I (DNase I) is an endonuclease that cleaves DNA by preferentially acting on phosphodiester bonds adjacent to pyrimidines, to produce polynucleotides with terminal 5`-phosphates. A tetranucleotide is the smallest average digestion product. DNase I hydrolyzes single and double-stranded DNA and chromatin (reaction rate is restricted by DNA association with histones). The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions. DNase I is found in most cells and tissues. In mammals, the pancreas is one of the best sources for the enzyme.
Application :
- Degradation of DNA template in transcription reactions
- Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
- Studies of DNA-protein interactions by DNase I, RNase-free footprinting
- Perform nick translations
Storage:
Store at –20 °C.
