DNase I (ready to use)
Features:
- Heat inactivation: DNase I is heat-inactivated by 5 minutes incubation at 75 °C
- Degrades double-stranded and single-stranded DNA
- EDTA chelates ions in the digestion buffer and inhibit the enzyme activation
| Brand |
YektaTajhiz Azma |
|---|
Deoxyribonuclease I (DNase I) is an endonuclease that cleaves DNA by preferentially acting on phosphodiester bonds adjacent to pyrimidines, to produce polynucleotides with terminal 5`-phosphates. A tetranucleotide is the smallest average digestion product. DNase I hydrolyzes single and double-stranded DNA and chromatin (reaction rate is restricted by DNA association with histones). The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions. DNase I is found in most cells and tissues. In mammals, the pancreas is one of the best sources for the enzyme.
Application :
- Degradation of DNA template in transcription reactions
- Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
- Studies of DNA-protein interactions by DNase I, RNase-free footprinting
- Perform nick translations
Storage:
Store at –20 °C.
Documents
protocol of DNase I (ready to use) → Download
Application :
- Degradation of DNA template in transcription reactions
- Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
- Studies of DNA-protein interactions by DNase I, RNase-free footprinting
- Perform nick translations
Storage:
Store at –20 °C
Accessibility
Contact us
Unit 21 , 9th floor ,( Rotano tower) No.218 , Resalat highway, Tehranpars , Tehran –Iran
